Lab Tests

Bong-Aid Third Party Lab Test

10366 Roselle St. Suite C • San Diego, CA 92121 • 858-535-9979 _______________Analytical Testing Report__________________ Bong Aid 05/12/2020 2901 W Coast Highway Suite 200 Newport Beach CA 92663 ECA# 20112 Purpose: Expert Chemical Analysis was contracted to assess the safety of using the raw material: Bong-Aid Proprietary Formula in a consumer product to be used in smoking devices. Customer Identification ECA ID Bong-Aid 20112a The analysis of the samples has been completed and the results are given below: _______________________________________________________________________ Notebook References TG007, pp. 110-112 Date Received: 4-14-20 Analysis Requested: 1. Modified USP <51>, Antimicrobial Effectiveness 2. Product Safety Opinion Date Tested: 04-14-20 to 05-12-20 Results are given on the following pages Conclusions: 1. Antimicrobial Effectiveness for Category 3 Products (Oral products, other than antacids made with aqueous-based vehicles) is verified per this test as there were no increases in counts of Aspergillus brasiliensis in 14 days or 28 days. 2. Bong-Aid Proprietary Formula appears to be safe for the intended use in this product. If you have any questions concerning these results, please call us at (858) 535-9979. We appreciate your business! Sincerely, Q.C. Officer Scientis1. Modified USP <51>, Antimicrobial Effectiveness Sample preparation: The microorganism —Aspergillus brasiliensis, is used to challenge the product for twenty-eight (28) days. Microorganism survival is monitored at seven (7), fourteen (14) and twenty-eight (28) days intervals. Results: Colony formation units (CFU/plate) Log reduction Micro organism Initial inoculum ECA# 0 hour 7 days 14 days 28 days 7 days 14 days 28 days A. brasiliensis 1x104 CFU/mL Control (PBS) 64 65 64 70 minimal change minimal change minimal change 20112 71 <1 <1 <1 1.85 1.85 1.85 2. Product Safety Opinion for Bong-Aid Proprietary Formula: Bong-Aid Proprietary Formula is commonly used in foods, beverages, and personal care items as an antimicrobial and preservative. Bong-Aid Proprietary Formula is GRAS (Generally Recognized as Safe) by the US. Food and Drug Association (FDA) for use as a food additive. It is also recognized as safe for use as a food additive by the United Nations Food and Agriculture Organization and the European Food Safety Authority. Bong-Aid Proprietary Formula is especially effective in inhibition of mold and yeast growth in food products and beverages. Bong-Aid Proprietary Formula has a maximum acceptable daily intake level of 1750 mg for an average sized adult. The LD50 in rats is 4350 mg/kg body weight. Bong-Aid Proprietary Formula is not a hazardous substance and is not a carcinogen, teratogen, or mutagen. For the reasons mentioned above, Bong-Aid Proprietary Formula appears to be very safe in this product for its intended use.